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Intra-day and inter-day precision of the method was characterised in terms of relative standard deviation (RSD, %). The limits of acceptable variability were set at 15% for all the concentrations, except at LLOQ, for which 20% was accepted. LLOD was defined as the lowest concentration of the analyte which gave a peak response equivalent to three times the background noise [i. LLOQ was defined as the lowest amount of analyte which gave a peak response with a S/N ? 10 and which could be measured with adequate precision and accuracy (RSD less than 20% and an inaccuracy '20%) [40]. The selectivity of the method was determined by analysing the drug-free samples of hair, urine and serum in replicates and confirming the absence of any detectable peaks at the retention times of stanozolol, 3 ? -hydroxystanozolol and ISs. 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